Stem Cell Therapy
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Stem Cells


This is a process that is used to preserve stem cell by cooling them at sub-zero temperatures for future use. This process is also used in infertility treatments to freeze sperms, embryos, and ova. The technique is still new and is yet to achieve perfection, though is highly popular among scientists to preserve samples of their research. It is used by hospitals and research institutes.


  1. Cryoprotectants- help in rapid freezing of the substance, turning it into a glass like state that does not have any crystal like formation. They protect the cells from getting killed and lower the freezing point. They also ensure that there is no intra cellular or extra cellular ice formation. The concentration of cryoprotectants is maintained based on the cells that have to be preserved. High concentration may result in harmful chemical reactions.

  2. Liquid nitrogen- This is the main agent in which the samples are stored. Since the boiling temperature of liquid nitrogen is extremely low (-195.8 º C), it is the perfect medium to carry out cryopreservation. Usually, the substances are stored at 77 K or -196˚C. The rate at which cooling tales place has to be maintained as improper cooling techniques can destroy the cells.

De-freezing /Thawing
Cryopreservation2 De-freezing or thawing is done rapidly using special techniques, so that the cells do not get damaged. Post this, the cells get coaxed into differentiating into a particular cell type and introduced into the patients body.

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